2QE5

Structure of HCV NS5B Bound to an Anthranilic Acid Inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-30
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	101.340, 101.465, 251.213
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 2.600
R-factor	0.218
Rwork	0.218
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1quv
RMSD bond length	0.008
RMSD bond angle	1.600
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	70034
<I/σ(I)>	7	3.4
Completeness [%]	87.2	79.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291	26% Polyehtylene Glycol 4000, 50 mM sodium acetate, pH 4.5 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K