2QE5
Structure of HCV NS5B Bound to an Anthranilic Acid Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.340, 101.465, 251.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.600 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1quv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 70034 | |
| <I/σ(I)> | 7 | 3.4 |
| Completeness [%] | 87.2 | 79.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 26% Polyehtylene Glycol 4000, 50 mM sodium acetate, pH 4.5 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
