2Q9Q
The crystal structure of full length human GINS complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97932, 0.97945 |
Spacegroup name | P 1 |
Unit cell lengths | 58.269, 89.096, 103.800 |
Unit cell angles | 105.02, 103.58, 95.07 |
Refinement procedure
Resolution | 29.580 - 2.360 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.24800 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.120 | |
Number of reflections | 143641 | |
Completeness [%] | 98.6 | 97.7 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 60mM MES (pH5.5),2% (v/v) isopropanol, 34mM calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 291K |