2Q9Q

The crystal structure of full length human GINS complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97932, 0.97945
Spacegroup name	P 1
Unit cell lengths	58.269, 89.096, 103.800
Unit cell angles	105.02, 103.58, 95.07

Refinement procedure

Resolution	29.580 - 2.360
R-factor	0.21
Rwork	0.210
R-free	0.24800
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.360	2.360
Rmerge	0.120
Number of reflections	143641
Completeness [%]	98.6	97.7
Redundancy		3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	60mM MES (pH5.5),2% (v/v) isopropanol, 34mM calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 291K