2Q8E
Specificity and Mechanism of JMJD2A, a Trimethyllysine-Specific Histone Demethylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2007-02-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 100.294, 148.964, 56.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.970 - 2.050 |
R-factor | 0.21501 |
Rwork | 0.213 |
R-free | 0.25966 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gp5 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.561 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.600 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.056 | 0.463 |
Number of reflections | 54092 | |
<I/σ(I)> | 11.4 | 3.1 |
Redundancy | 5.8 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |