2Q8E

Specificity and Mechanism of JMJD2A, a Trimethyllysine-Specific Histone Demethylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	200
Detector technology	CCD
Collection date	2007-02-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	100.294, 148.964, 56.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.970 - 2.050
R-factor	0.21501
Rwork	0.213
R-free	0.25966
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gp5
RMSD bond length	0.015
RMSD bond angle	1.561
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.600	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.056	0.463
Number of reflections	54092
<I/σ(I)>	11.4	3.1
Redundancy	5.8	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1