2Q8C
Crystal structure of JMJD2A in ternary complex with an histone H3K9me3 peptide and 2-oxoglutarate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2007-01-30 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 100.887, 149.121, 57.056 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.900 - 2.047 |
R-factor | 0.20296 |
Rwork | 0.201 |
R-free | 0.23584 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gp5 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.253 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.900 | 2.120 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.045 | 0.506 |
Number of reflections | 54585 | |
<I/σ(I)> | 39 | 2.3 |
Redundancy | 5.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |