2PVC
DNMT3L recognizes unmethylated histone H3 lysine 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2006-10-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 267.700, 267.700, 150.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.410 - 3.690 |
| R-factor | 0.264 |
| Rwork | 0.264 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pv0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.830 |
| High resolution limit [Å] | 3.690 | 3.690 |
| Rmerge | 0.105 | 0.496 |
| Number of reflections | 30684 | |
| <I/σ(I)> | 11.6 | 2.8 |
| Completeness [%] | 89.4 | 89.4 |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 300 | 12 % polyethylene glycol, 0.2 M sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K, pH 7.50 |
