2PNX
The PHD finger of ING4 in complex with an H3K4Me3 histone peptide
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-22 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.282, 1.283, 1.257 |
| Spacegroup name | P 43 |
| Unit cell lengths | 68.160, 68.160, 27.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.080 - 1.800 |
| Rwork | 0.199 |
| R-free | 0.21100 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.600 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.080 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.089 | 0.244 |
| Number of reflections | 22220 | |
| <I/σ(I)> | 13 | 2.2 |
| Completeness [%] | 98.8 | 90.1 |
| Redundancy | 6.69 | 3.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | 1.6 M sodium citrate tribasic dihydrate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |
