2PJY
Structural basis for cooperative assembly of the TGF-beta signaling complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-06 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 66.920, 66.920, 254.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.800 - 3.000 |
R-factor | 0.244 |
Rwork | 0.242 |
R-free | 0.29700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ktz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.809 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.290 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.074 | 0.598 |
Number of reflections | 6710 | |
<I/σ(I)> | 26.2 | 4.2 |
Completeness [%] | 90.3 | 100 |
Redundancy | 8.7 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 10-20% PEG 3350, 0.4-0.65 M calcium acetate, 0.1-0.25M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |