2PBI
The multifunctional nature of Gbeta5/RGS9 revealed from its crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-23 |
Detector | MARRESEARCH |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 110.167, 119.032, 131.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.950 |
R-factor | 0.185 |
Rwork | 0.183 |
R-free | 0.22500 |
Structure solution method | MIRAS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.155 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
Rmerge | 0.075 | 0.060 | 0.434 |
Number of reflections | 125677 | ||
<I/σ(I)> | 13.6 | ||
Completeness [%] | 99.9 | 99.6 | 99.7 |
Redundancy | 4.1 | 4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 291 | MME PEG 550, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 291 | MME PEG 550, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 291 | MME PEG 550, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 291 | MME PEG 550, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 291 | MME PEG 550, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 291 | MME PEG 550, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 291K |