2P2C
Inhibition of caspase-2 by a designed ankyrin repeat protein (DARPin)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.899 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.020, 229.210, 114.930 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.240 |
| Rwork | 0.262 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1PYO and 1MJO |
| RMSD bond length | 1.905 |
| RMSD bond angle | 3.378 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.500 |
| High resolution limit [Å] | 3.240 | 3.240 |
| Rmerge | 0.140 | 0.690 |
| Number of reflections | 92159 | |
| <I/σ(I)> | 9.81 | 2.11 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 1.79 | 1.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 17% PEG 5000-MME, 0.1 M Tris-HOAc, 0.1 M KSCN, 30% ethylene glycol, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
