2OZA
Structure of p38alpha complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-29 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 83.310, 83.310, 231.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.800 - 2.700 |
Rwork | 0.239 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.094 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.800 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Total number of observations | 12295 | |
Number of reflections | 22784 | |
<I/σ(I)> | 8.8 | 2.1 |
Completeness [%] | 98.0 | 84.9 |
Redundancy | 7.6 | 5.74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 5 to 20% PEG 4000, 100mM Na Citrate, 5mM DTT, pH 6.0, vapor diffusion, hanging drop, temperature 298K |