2OYM
Endo-glycoceramidase II from Rhodococcus sp.: five-membered iminocyclitol complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.786, 93.282, 94.471 |
| Unit cell angles | 90.00, 98.30, 90.00 |
Refinement procedure
| Resolution | 18.300 - 1.860 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2osw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.196 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.300 | 1.950 |
| High resolution limit [Å] | 1.841 | 1.841 |
| Rmerge | 0.048 | 0.346 |
| Total number of observations | 33486 | |
| Number of reflections | 72622 | |
| <I/σ(I)> | 14.1 | 2.2 |
| Completeness [%] | 91.9 | 75.4 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 20% (w/v) PEG 3350; 0.175 M NaCl; 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
