2ONL
Crystal Structure of the p38a-MAPKAP kinase 2 Heterodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 95 |
Spacegroup name | P 41 |
Unit cell lengths | 103.150, 103.150, 231.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.320 | 4.140 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.153 | 0.368 |
Number of reflections | 19566 | |
<I/σ(I)> | 5.1 | 2.1 |
Completeness [%] | 95.8 | 95.8 |
Redundancy | 2.79 | 2.66 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 3.75% Peg 2000, 100mM Hepes, Prior to crystallization, 1-s-nonyl-1-b-D-thioglucoside (1xcmc) and heptanetriol (1.5%) was added to the protein sample, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.50 |