2OM2

Crystal Structure Of Human G[alpha]i1 Bound To The Goloco Motif Of Rgs14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-18
Detector	MARRESEARCH
Spacegroup name	P 2 2 21
Unit cell lengths	69.592, 82.311, 187.566
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
Rwork	0.227
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kjy
RMSD bond length	0.006
RMSD bond angle	1.130
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.200
Number of reflections	55436
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	1.55M Ammonium Sulfate, 100 mM Sodium Acetate pH 5.0, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K