2OAX
Crystal structure of the S810L mutant mineralocorticoid receptor associated with SC9420
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 81 |
Detector technology | CCD |
Collection date | 2005-02-05 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979707 |
Spacegroup name | P 32 |
Unit cell lengths | 122.198, 122.198, 91.808 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.920 - 2.290 |
R-factor | 0.24 |
Rwork | 0.240 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | monomere A from PDB 1Y9R |
RMSD bond length | 0.014 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.920 | 2.430 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.121 | 0.623 |
Number of reflections | 63172 | |
<I/σ(I)> | 13 | 2.66 |
Completeness [%] | 91.1 | 94.8 |
Redundancy | 6.18 | 5.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 297 | HEPES 100 mM, pH 6.8, 230 mM NaCl, 25% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 297K |