2O3C
Crystal structure of zebrafish Ape
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-22 |
| Detector | NOIR-1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.660, 117.780, 85.840 |
| Unit cell angles | 90.00, 98.34, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bix |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.380 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.059 | 0.268 |
| Number of reflections | 46075 | |
| <I/σ(I)> | 14.3 | 3.4 |
| Completeness [%] | 96.5 | 85.5 |
| Redundancy | 3.07 | 2.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 290 | AMMONIUM ACETATE, BIS-TRIS, PEG 3350, GLYCEROL, LEAD ACETATE, PH 5.5, pH 5.50, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
