2O2K
Crystal Structure of the Activation Domain of Human Methionine Synthase Isoform/Mutant D963E/K1071N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2005-12-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.854, 90.051, 123.006 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 1.600 |
| R-factor | 0.2113 |
| Rwork | 0.209 |
| R-free | 0.24782 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1msk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.038 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 106145 | |
| <I/σ(I)> | 20.1 | 3.2 |
| Completeness [%] | 96.3 | 96.5 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 0.1M Tris/HCl, 0.1-0.4M sodium acetate, 10-12% PEG 8000, 10-12% PEG 1000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 292.0K |
