2O1S
1-deoxy-D-xylulose 5-phosphate synthase (DXS) from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-06 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.787, 171.154, 94.799 |
| Unit cell angles | 90.00, 107.25, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.081 | 0.273 |
| Number of reflections | 91600 | |
| <I/σ(I)> | 12.5 | 4.5 |
| Completeness [%] | 92.8 | 83.8 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 30% (w/v) PEG 3350,200 mM Na/K tartrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
