2O1S
1-deoxy-D-xylulose 5-phosphate synthase (DXS) from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-06 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.787, 171.154, 94.799 |
Unit cell angles | 90.00, 107.25, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.23400 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.081 | 0.273 |
Number of reflections | 91600 | |
<I/σ(I)> | 12.5 | 4.5 |
Completeness [%] | 92.8 | 83.8 |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 30% (w/v) PEG 3350,200 mM Na/K tartrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |