Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-08-24 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.200, 116.205, 55.993 |
Unit cell angles | 90.00, 90.46, 90.00 |
Refinement procedure
Resolution | 58.120 - 2.060 |
R-factor | 0.202 |
Rwork | 0.199 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b4y |
RMSD bond length | 0.008 |
RMSD bond angle | 1.730 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.120 | 2.100 |
High resolution limit [Å] | 2.060 | 2.060 |
Number of reflections | 28096 | |
Completeness [%] | 86.4 | 86.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 25% PEG 3350, 200mM NaCl, 0.1M Tris-HCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |