2NT1
Structure of acid-beta-glucosidase at neutral pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-30 |
Detector | MARRESEARCH |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 110.185, 91.792, 153.036 |
Unit cell angles | 90.00, 110.91, 90.00 |
Refinement procedure
Resolution | 19.940 - 2.300 |
R-factor | 0.178 |
Rwork | 0.175 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nt0 |
RMSD bond length | 0.022 |
RMSD bond angle | 1.969 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.454 | |
Number of reflections | 119558 | |
<I/σ(I)> | 7.8 | 2.4 |
Completeness [%] | 95.1 | 79.6 |
Redundancy | 3.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.8 M Na dihydrogen phosphate 0.8 M K dihydrogen phosphate 0.1 M Hepes buffer pH 7.5 Cryoprotected in 2 M Lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |