2NSX
Structure of acid-beta-glucosidase with pharmacological chaperone provides insight into Gaucher disease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.967, 92.216, 152.670 |
| Unit cell angles | 90.00, 110.94, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.110 |
| R-factor | 0.19319 |
| Rwork | 0.191 |
| R-free | 0.23492 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ogs |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.527 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 149310 | |
| <I/σ(I)> | 13.1 | 3 |
| Completeness [%] | 91.5 | 70.4 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 1 M Ammonium Sulfate 0.17 M Guanidinium HCl 0.02 M KCl 0.1 M Acetate buffer pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
