2NS7
How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-22 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.946452 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 107.910, 107.910, 303.940 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.040 - 2.400 |
R-factor | 0.234 |
Rwork | 0.230 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2NS8 (Chain A+B) |
RMSD bond length | 0.010 |
RMSD bond angle | 1.202 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.040 | 2.450 | |
High resolution limit [Å] | 2.400 | 4.800 | 2.400 |
Rmerge | 0.061 | 0.051 | 0.472 |
Number of reflections | 41266 | ||
<I/σ(I)> | 28.43 | 60.9 | 4.9 |
Completeness [%] | 98.4 | 98.9 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 1.4M ammonium sulfate, 0.1M sodium chloride, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |