2N7A

Solution structure of the human Siglec-8 lectin domain

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE	Bruker Avance	500
2	Bruker	AVANCE	Bruker Avance	600
3	Bruker	AVANCE	Bruker Avance	700
4	Bruker	AVANCE	Bruker Avance	750
5	Bruker	AVANCE	Bruker Avance	900

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	2	3D 1H-15N NOESY
11	1	5	2D 1H-1H NOESY
12	1	2	3D 1H-13C NOESY aliphatic
13	1	2	3D 1H-13C NOESY aromatic
14	1	5	2D 1H-1H TOCSY
15	1	3	2D 1H-13C constant-time HSQC to obtain stereospecific assignments of Val/Leu methyls
2	1	2	2D 1H-13C HSQC aliphatic
3	1	2	2D 1H-13C HSQC aromatic
4	1	2	3D HNCA
5	1	2	3D HNCACB
6	1	2	3D CBCA(CO)NH
7	1	2	3D HNCO
8	1	2	3D HN(CO)CA
9	1	4	3D HCCH-TOCSY

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	7	ambient	293

Conformers

Conformers Calculated Total Number	500
Conformers Submitted Total Number	20