2LMS
A single GalNAc residue on Threonine-106 modifies the dynamics and the structure of Interferon alpha-2a around the glycosylation site
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | Bruker Avance | 700 |
| 2 | Bruker | AVANCE III | Bruker Avance III | 600 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 3D HNCO |
| 10 | 1 | 1 | 3D C(CO)NH |
| 11 | 1 | 2 | 2D (hb)CB(cgcd)HD |
| 12 | 1 | 2 | 2D (hb)CB(cgcdce)HE |
| 13 | 1 | 1 | 4D (C13)HSQC-NOESY-(N15)HSQC |
| 14 | 1 | 2 | 4D (C13)HSQC-NOESY-(C13)HSQC |
| 2 | 1 | 1 | 3D HNCA |
| 3 | 1 | 1 | 3D HNCACB |
| 4 | 1 | 1 | 3D CBCA(CO)NH |
| 5 | 1 | 1 | 3D HBHA(CO)NH |
| 6 | 1 | 1 | 3D HCCH-TOCSY |
| 7 | 1 | 1 | 3D 1H-15N NOESY |
| 8 | 1 | 2 | 3D 1H-13C NOESY aliphatic |
| 9 | 1 | 2 | 3D 1H-13C NOESY aromatic |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 3.5 | ambient | 298.15 |
Conformers
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
