2LI8
The solution structure of the Lin28-ZnF domains bound to AGGAGAU of pre-let-7 miRNA
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AV-III | Bruker AV-III | 500 |
| 2 | Bruker | AV-III | Bruker AV-III | 600 |
| 3 | Bruker | AV-III | Bruker AV-III | 700 |
| 4 | Bruker | Avance | Bruker Avance | 900 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 2 | 3D_15N-separated_NOESY 3D_13C-separated_NOESY |
| 10 | 1 | 3 | 3D HCCH-TOCSY |
| 11 | 1 | 3 | 2D 1H-1H TOCSY |
| 12 | 1 | 3 | 2D-F1f-F2f-NOESY |
| 13 | 1 | 3 | 2D-F2f-NOESY |
| 14 | 1 | 3 | 3D-F3f-NOESY |
| 15 | 1 | 2 | 2D 1H-15N HMQC |
| 16 | 1 | 2 | 2D 1H-1H NOESY |
| 17 | 2 | 2 | 2D 1H-1H NOESY |
| 18 | 1 | 4 | 2D 1H-13C HSQC |
| 19 | 2 | 1 | 3D-F3fe-NOESY |
| 2 | 1 | 1 | 3D 1H-13C NOESY |
| 20 | 2 | 2 | 3D 1H-15N NOESY |
| 3 | 1 | 1 | 2D 1H-15N HSQC |
| 4 | 1 | 3 | 2D 1H-13C HSQC aliphatic |
| 5 | 1 | 3 | 2D 1H-13C HSQC aromatic |
| 6 | 1 | 1 | 3D CBCA(CO)NH |
| 7 | 1 | 3 | 3D HNCACB |
| 8 | 1 | 1 | 3D HNCO |
| 9 | 1 | 1 | 3D H(CCO)NH |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 5.6 | 1 | 303 |
| 2 | 5.6 | 1 | 283 |
Conformers
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 20 |
