2JCC
AH3 recognition of mutant HLA-A2 W167A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.280, 84.346, 122.470 |
| Unit cell angles | 90.00, 92.53, 90.00 |
Refinement procedure
| Resolution | 122.170 - 2.500 |
| R-factor | 0.244 |
| Rwork | 0.242 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lp9 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.585 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.670 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.380 |
| Number of reflections | 152000 | |
| <I/σ(I)> | 9 | 2 |
| Completeness [%] | 92.7 | 85 |
| Redundancy | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
