2J59

Crystal structure of the ARF1:ARHGAP21-ArfBD complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-09
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	74.636, 132.136, 146.283
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	40.000 - 2.100
R-factor	0.209
Rwork	0.205
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1J2I
RMSD bond length	0.009
RMSD bond angle	1.203
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.090	0.370
Number of reflections	155860
<I/σ(I)>	14.6	3.8
Completeness [%]	98.4	95.5
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.8		15 % PEG5000 MME, 100 MM IMIDAZOLE PH 6.8, 150 MM NH4.SO4 OR LI2SO4, 10 MM MGCL2, 0.5 % DIOXANE, 5 % ETHYLENE GLYCOL