2J0U

The crystal structure of eIF4AIII-Barentsz complex at 3.0 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-17
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	71.760, 107.190, 243.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.000
R-factor	0.276
Rwork	0.273
R-free	0.32800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fuu 1fuk 1p27
RMSD bond length	0.011
RMSD bond angle	1.436
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.200
High resolution limit [Å]	3.000	3.000
Rmerge	0.220	0.610
Number of reflections	18221
<I/σ(I)>	4.3	2
Completeness [%]	94.2	86.4
Redundancy	3	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				700 MM DI AMMONIUM TARTRATE, 100 MM NA ACETATE PH 4.6