2J0T
Crystal Structure of the Catalytic Domain of MMP-1 in Complex with the Inhibitory Domain of TIMP-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-23 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.098, 67.850, 86.241 |
| Unit cell angles | 90.00, 100.29, 90.00 |
Refinement procedure
| Resolution | 23.000 - 2.540 |
| R-factor | 0.249 |
| Rwork | 0.248 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1CGL AND 1UEA |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.892 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.630 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.090 | 0.450 |
| Number of reflections | 29526 | |
| <I/σ(I)> | 8.3 | 2.8 |
| Completeness [%] | 98.4 | 99.4 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10% PEG 8000, 8% ETHYLENE GLYCOL, 0.1M HEPES (PH 7.5). |
