2IYK
Crystal structure of the UPF2-interacting domain of nonsense mediated mRNA decay factor UPF1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.560, 72.980, 73.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.710 - 2.950 |
R-factor | 0.223 |
Rwork | 0.221 |
R-free | 0.25900 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.229 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.000 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.120 | 0.420 |
Number of reflections | 7000 | |
<I/σ(I)> | 10.2 | 2.9 |
Completeness [%] | 90.9 | 96.5 |
Redundancy | 4.9 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 30% PENTAERYTHRITOL ETHOXYLATE (15/4EO/OH) (V/V), 50 MM AMMONIUM SULPHATE,50 MM BIS-TRIS PH 6.5 |