2IYF
The crystal structure of macrolide glycosyltransferases: A blueprint for antibiotic engineering
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2006-02-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.718, 65.778, 91.940 |
| Unit cell angles | 90.00, 100.42, 90.00 |
Refinement procedure
| Resolution | 29.880 - 1.700 |
| R-factor | 0.201 |
| Rwork | 0.200 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.513 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.410 |
| Number of reflections | 94042 | |
| <I/σ(I)> | 16.06 | 2.82 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20-23% PEG8K,0.2M MGCL2,0.1M TRIS-HCL PH 8.5 |
