2IXE
Crystal structure of the ATPase domain of TAP1 with ATP (D645N mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2005-08-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 80.431, 80.431, 168.115 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.550 - 2.000 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jj7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.094 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 38086 | |
| <I/σ(I)> | 26.6 | 3 |
| Completeness [%] | 99.7 | 97.9 |
| Redundancy | 9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | HANGING DROP VAPOR DIFFUSION AT 4C WITH A RESERVOIR CONTAING 1.0 M POTASSIUM PHOSPHATE PH 8.0 |
