2IWD
Oxacilloyl-acylated MecR1 extracellular antibiotic-sensor domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.970, 57.970, 147.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iwc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.566 |
| Data reduction software | XDS |
| Data scaling software | COMBAT |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.070 |
| Number of reflections | 10438 | |
| <I/σ(I)> | 21.5 | 4.6 |
| Completeness [%] | 99.5 | 97.6 |
| Redundancy | 7.1 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.8M TRIBASIC AMMONIUM CITRATE AT PH6.5, pH 6.50 |
