2IUW
Crystal structure of human ABH3 in complex with iron ion and 2- oxoglutarate
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-12 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 |
| Unit cell lengths | 119.955, 119.955, 40.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 1.500 |
| R-factor | 0.1406 |
| R-free | 0.19120 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.029 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.100 | 0.470 |
| Number of reflections | 46030 | |
| <I/σ(I)> | 43.4 | 4.5 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 7.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M BIS-TRIS PH 5.5, 0.1 M (NH4)2SO4, 4-9 % PEG 8000/10000 |
