2IO4

Crystal structure of PCNA12 dimer from Sulfolobus solfataricus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	104.991, 112.727, 101.851
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.234
Rwork	0.233
R-free	0.26036
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.349
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.660
High resolution limit [Å]	2.600	2.600
Rmerge	0.058	0.728
Number of reflections	37787
<I/σ(I)>	27.5	1.92
Completeness [%]	99.8	99.8
Redundancy	5.2	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% MPD, 20 mM CaCl2, 0.1 M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% MPD, 20 mM CaCl2, 0.1 M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% MPD, 20 mM CaCl2, 0.1 M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K