2IO4
Crystal structure of PCNA12 dimer from Sulfolobus solfataricus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 104.991, 112.727, 101.851 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.234 |
Rwork | 0.233 |
R-free | 0.26036 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.349 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.660 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.058 | 0.728 |
Number of reflections | 37787 | |
<I/σ(I)> | 27.5 | 1.92 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 5.2 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 25% MPD, 20 mM CaCl2, 0.1 M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 25% MPD, 20 mM CaCl2, 0.1 M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 25% MPD, 20 mM CaCl2, 0.1 M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |