2IM2
Crystal structure of poliovirus polymerase complexed with UTP and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 |
Unit cell lengths | 128.981, 128.981, 113.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.000 - 2.350 |
Rwork | 0.229 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ra6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 43793 | |
<I/σ(I)> | 16.6 | 4.6 |
Completeness [%] | 98.5 | 97.7 |
Redundancy | 7.15 | 7.07 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | sodium acetate, cacodylate, DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |