2IM2

Crystal structure of poliovirus polymerase complexed with UTP and Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 65
Unit cell lengths	128.981, 128.981, 113.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.000 - 2.350
Rwork	0.229
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ra6
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	43793
<I/σ(I)>	16.6	4.6
Completeness [%]	98.5	97.7
Redundancy	7.15	7.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	sodium acetate, cacodylate, DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K