2IHB

Crystal structure of the heterodimeric complex of human RGS10 and activated Gi alpha 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97925
Spacegroup name	P 43 21 2
Unit cell lengths	100.628, 100.628, 145.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.600 - 2.710
R-factor	0.20913
Rwork	0.208
R-free	0.23632
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Swissmodel based on the coordinates of PDB entries 1agr 1ezt 1ezy 2bv1
RMSD bond length	0.009
RMSD bond angle	1.057
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Number of reflections	20470
Completeness [%]	97.2	80.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.2M (NH4)2SO4, 0.1M Bis-Tris, pH 5.5, 25% PEG3350 , VAPOR DIFFUSION, SITTING DROP, temperature 293K