2IEJ
Human Protein Farnesyltransferase Complexed with Inhibitor Compound STN-48 And FPP Analog at 1.8A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 61 |
| Unit cell lengths | 178.253, 178.253, 64.661 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.690 - 1.800 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.294 |
| Data reduction software | HKL-2000 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 | |
| High resolution limit [Å] | 1.800 | 6.000 | 1.800 |
| Rmerge | 0.041 | 0.018 | 0.271 |
| Number of reflections | 107537 | ||
| <I/σ(I)> | 22.72 | 59.6 | 3.7 |
| Completeness [%] | 97.6 | 99.3 | 88.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | crystallized via hanging drop against well solution containing 300 mM ammonium acetate pH 5.2, 12% PEG 8K , VAPOR DIFFUSION, HANGING DROP |
