2IC5
Crystal structure of human RAC3 grown in the presence of Gpp(NH)p.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.755, 81.583, 84.381 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.310 - 1.900 |
| R-factor | 0.15505 |
| Rwork | 0.153 |
| R-free | 0.20026 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g0n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.469 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.310 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 29632 | |
| Completeness [%] | 98.8 | 92.3 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 0.1 M BIS-TRIS, 20% MPEG 5K , pH 6.5, temperature 293K |
