2I3U
Structural studies of protein tyrosine phosphatase beta catalytic domain in complex with inhibitors
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.785, 69.777, 118.523 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.310 - 1.850 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i3r |
RMSD bond length | 0.010 |
RMSD bond angle | 1.194 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.310 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.028 | 0.190 |
Number of reflections | 27971 | |
<I/σ(I)> | 21.75 | 5.11 |
Completeness [%] | 98.2 | 93.3 |
Redundancy | 4.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 278 | 21% PEG 8000, 220 mM MgCl2, 1% BME, 0.1% BOG, 5mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 278K |