2I2Q
Fission Yeast cofilin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-26 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.915, 81.915, 61.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.720 |
| R-factor | 0.241 |
| Rwork | 0.236 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cof |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.071 | |
| Number of reflections | 25210 | |
| <I/σ(I)> | 18.5 | |
| Completeness [%] | 88.8 | 35.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 296 | 2.1 M Ammonium Sulfate, 100 mM imidazole pH 7.0, 1 mM NiSO4, 2.5% PEG 400, 1 mM LDAO, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
