2I0E
Structure of catalytic domain of human protein kinase C beta II complexed with a bisindolylmaleimide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.098, 131.417, 83.801 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.23654 |
| Rwork | 0.235 |
| R-free | 0.28973 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Akt-1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.329 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.049 | |
| Number of reflections | 32375 | |
| <I/σ(I)> | 28.4 | 3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 286 | 0.1 M ADA, 2.5 M sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 286K |
