2I0B
Crystal structure of the GluR6 ligand binding core ELKQ mutant dimer at 1.96 Angstroms Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-19 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 94.215, 94.215, 219.836 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.960 |
R-factor | 0.17404 |
Rwork | 0.172 |
R-free | 0.21055 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 17SY |
RMSD bond length | 0.015 |
RMSD bond angle | 1.671 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.790 | 2.030 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.058 | 0.452 |
Number of reflections | 68095 | |
<I/σ(I)> | 13 | 3.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.5 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | Reservoir: 1.5 M Ammonium Sulfate, 0.1 M NaCl, 0.1 M HEPES. 0.015 M Na Acetate; Protein: 12-22 mg/ml, 10 mM L-glutamate, 20 mM NaCl, 1 mM EDTA, pH 7.0, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |