2I0B

Crystal structure of the GluR6 ligand binding core ELKQ mutant dimer at 1.96 Angstroms Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-19
Detector	MARRESEARCH
Wavelength(s)	1.00000
Spacegroup name	P 43 21 2
Unit cell lengths	94.215, 94.215, 219.836
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.960
R-factor	0.17404
Rwork	0.172
R-free	0.21055
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	17SY
RMSD bond length	0.015
RMSD bond angle	1.671
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.790	2.030
High resolution limit [Å]	1.960	1.960
Rmerge	0.058	0.452
Number of reflections	68095
<I/σ(I)>	13	3.8
Completeness [%]	99.9	100
Redundancy	6.5	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	Reservoir: 1.5 M Ammonium Sulfate, 0.1 M NaCl, 0.1 M HEPES. 0.015 M Na Acetate; Protein: 12-22 mg/ml, 10 mM L-glutamate, 20 mM NaCl, 1 mM EDTA, pH 7.0, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K