2HZP
Crystal Structure of Homo Sapiens Kynureninase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.117, 76.838, 93.274 |
| Unit cell angles | 90.00, 108.70, 90.00 |
Refinement procedure
| Resolution | 88.390 - 2.000 |
| R-factor | 0.15217 |
| Rwork | 0.150 |
| R-free | 0.19534 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qz9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.135 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.390 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.055 | 0.264 |
| Number of reflections | 33266 | |
| <I/σ(I)> | 24.68 | 5.046 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under oil | 8 | 298 | A 9.2 mg/ml kynureninase solution in 50 mM HEPES pH 5.2, 0.2 mM PLP was mixed in equal parts with 0.1 M Tris-Cl pH 8.0, 0.05 M MgCl2, 25% PEG 3000, Microbatch under oil, temperature 298K |
