2HZ6
The crystal structure of human IRE1-alpha luminal domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0023, 0.9785, 0.9795, 0.9567 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 185.010, 185.010, 67.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.100 |
| R-factor | 0.268 |
| Rwork | 0.268 |
| R-free | 0.31600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 12651 | |
| Completeness [%] | 98.5 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 3-3.5% PEG 20000, 0.1M PIPES, 3% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
