2HV8
Crystal structure of GTP-bound Rab11 in complex with FIP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-18 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 218.291, 52.601, 70.679 |
| Unit cell angles | 90.00, 105.56, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.860 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1YZK |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.972 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 64451 | |
| <I/σ(I)> | 27.5 | 5 |
| Completeness [%] | 97.1 | 77 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20% PEG4000, 0.4M Ammonium Sulfate, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
