2HUE
Structure of the H3-H4 chaperone Asf1 bound to histones H3 and H4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2005-09-30 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.000, 1.23 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 95.749, 95.749, 110.676 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 11.980 - 1.700 |
| Rwork | 0.209 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.556 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 12.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 61200 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1M potassium sulfate, 0.1M Tris-HCl, 14.5% PEG 4K, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
