2HRT
Asymmetric structure of trimeric AcrB from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-09 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 |
| Unit cell lengths | 127.330, 134.870, 140.840 |
| Unit cell angles | 103.90, 94.64, 90.11 |
Refinement procedure
| Resolution | 29.800 - 3.000 |
| R-factor | 0.234 |
| Rwork | 0.231 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iwg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.059 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.134 | 0.763 |
| Number of reflections | 181133 | |
| <I/σ(I)> | 8.4 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 290 | 5 % polyethylene glycol 400, 16-22 % polyethylene glycol 300, 8-11 % glycerol, 70 mM Nacitrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 4.60 |
