2HL5
Crystal structure of the C-terminal domain of human EB1 in complex with the A49M mutant CAP-Gly domain of human Dynactin-1 (p150-Glued)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-01-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97900 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 86.409, 101.304, 40.047 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.800 - 1.930 |
R-factor | 0.21237 |
Rwork | 0.211 |
R-free | 0.24185 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.221 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.800 | 1.980 |
High resolution limit [Å] | 1.930 | 1.930 |
Number of reflections | 26892 | |
Completeness [%] | 98.8 | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 25% PEG 3350, 0.1M HEPES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |