2HKJ
Topoisomerase VI-B bound to radicicol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2005-05-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1157 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.879, 111.398, 54.888 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.17025 |
| Rwork | 0.167 |
| R-free | 0.20877 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mu5 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.410 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 39306 | |
| <I/σ(I)> | 10.5 | 2.2 |
| Completeness [%] | 99.0 | 94.6 |
| Redundancy | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 292 | HEPES, Mg(HCO2)2, PEG 3350, NaCl, MgCl2, pH 7.5, microbatch, temperature 292K |
