2HEV

Crystal structure of the complex between OX40L and OX40

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-26
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9999
Spacegroup name	H 3 2
Unit cell lengths	111.903, 111.903, 233.238
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.410
R-factor	0.21248
Rwork	0.208
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.109
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	22255
<I/σ(I)>	11.8	2.3
Completeness [%]	99.9	100
Redundancy	6.1	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	8% PEG 20000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K